Time of day impacts virtually all aspects of life, including death Processes as fundamental as sleep, locomotion, cognitive function, feeding, digestion/absorption, body temperature, neural activity, endocrine factor release, and cardiovascular function all exhibit time-of-day-dependent oscillations.1,2 In terms of the cardiovascular system, both physiological (e.g., blood pressure, heart rate, cardiac output) and pathophysiological (e.g., adverse ischemic events, arrhythmias, sudden cardiac death) parameters vary over the course of the day.3 It is therefore not surprising that at biologic/molecular levels, cardiovascular components are markedly different during the day versus the night.
In the case of the heart, unbiased transcriptome and proteome analyses reveal an array of gene/protein expression differences as a function of time of day, thereby influencing a plethora of cellular processes.4,5 In addition, candidate signaling approaches suggest daily oscillations in the heart's sensitivity to extracellular stimuli such as fatty acids, -adrenergic agonist, and thyroid hormone.69 The focus of this review is on circadian control of cardiac metabolism, including the mechanisms involved in synchronizing metabolic rhythms, their role in maintaining cardiac function, and the pathological consequences of their disruption/impairment.
Synchronization of Metabolic Rhythms
Time-of-day-dependent oscillations in metabolism have been described at various levels in organisms ranging from yeast (e.g., NAD/NADH ratio) to humans (e.g., body temperature and energy expenditure).10 Evolutionarily speaking, such oscillations are predicted to confer a selective advantage, allowing the organism to adapt to daily fluctuations in the environmentfor example, lighting, temperature, humidity, food availability, and likelihood of predator interaction. At a biological level, it is therefore important to synchronize metabolic processes with time-of-day-dependent perturbations in energetic demand and nutrient availability that are associated with normal light/dark, sleep/wake, and fasting/feeding cycles. In mammals, such a synchronization must occur at intra- and inter-organ levels.
Classically, a stimulus-response model has been proposed wherein neurohumoral changes orchestrate oscillations in metabolic processes between cells/organs. With insulin, for example, increased secretion postprandially promotes peripheral tissue glucose utilization concomitant with attenuation of hepatic glucose production, thus facilitating glucose homeostasis by maintaining blood glucose within a physiologic range.11 What has become increasingly apparent is that cells/organs/organisms possess an ability to predict daily metabolic perturbations before they occur. In doing so, anticipation allows a temporally appropriate and rapid response to a stimuli once it occurs. Again, considering post-prandial insulin-mediated glucose utilization, the amplitude of both insulin secretion and sensitivity differs as a function of time of day due to anticipatory mechanisms.10
The process of anticipation is undoubtedly conferred by circadian clocks. Circadian clocks are cell-autonomous, transcriptionally based molecular mechanisms composed of a series of positive and negative feedback loops with a periodicity of approximately 24 hours. Central to the mechanism are BMAL1 and CLOCK, two transcription factors that, upon heterodimerization, bind to E-boxes within the promoter of target genes. The latter include multiple period and cryptochrome isoforms as well as REV-ERB, which translocate back into the nucleus and inhibit the BMAL1/CLOCK heterodimer (i.e., feedback inhibition).1 In addition, these transcription factors affect expression of a large number of genes that are not core clock components, also known as clock controlled genes (CCGs). By modulating the expression levels of CCGs in a time-of-day-dependent manner, the clock has the potential of temporally regulating a host of cellular processes such as transcription, translation, signal transduction, ion homeostasis, and metabolism.
A key element for maintaining this selective advantage is entrainment; cellular clocks must be synchronized with the environment. Within the hypothalamus, a region of approximately 20,000 specialized neurons known as the suprachiasmatic nucleus (SCN) receives light signals via the retinohypothalamic tract, resulting in entrainment. The SCN, often termed the master clock, subsequently sends neurohumoral signals to other tissues, thus resetting slave clocks. It is noteworthy that non-SCN clocks can also be entrained through common behaviors such as eating and physical activity. These entrainment mechanisms ensure that cell autonomous clocks can maintain synchrony between environmental/behavioral-associated factors (e.g., energetic demand and nutrient availability) and biological processes such as metabolism.
Cardiac Metabolic Rhythms
With respect to metabolism, the heart has two 24-hr cycles: sleep/wake and fasting/feeding. Importantly, the heart must meet increased energetic demands even if the animal in the wild is unsuccessful in its forage for food, as the animal continues to forage and avoid predation. It is therefore not surprising that dramatic fluctuations in cardiac metabolism have been reported at various levels, including daily rhythms in cardiac glucose, fatty acid, and amino acid utilization.6,1216 During periods of increased physical activity, the heart relies primarily on augmented glucose utilization to meet energetic needs for force generation and ion homeostasis.17,18 Consistent with this concept, myocardial glycolysis and glucose oxidation both increase during the awake phase (relative to the sleep phase; Figure 1 A).12,15 Importantly, these rhythms in glucose metabolism are observed in ex vivo perfused rat and mouse hearts, wherein both the milieu and workload are maintained at a constant level. This suggests that they are mediated, at least in part, by an intrinsic mechanism. A likely candidate is the cardiomyocyte circadian clock, as genetic ablation of this mechanism markedly attenuates/abolishes myocardial glucose metabolism rhythms.15,19 Collectively, these observations have led to the concept that the cardiomyocyte circadian clock allows anticipation of increased energetic demand during the awake period through modulation of glucose utilization.
Diurnal variations in cardiac metabolism extend beyond glucose catabolism. Turnover (i.e., synthesis/degradation cycles) of glycogen, triglycerides, and protein exhibit time-of-day-dependence in rodent hearts. In the cases of glycogen and triglyceride synthesis, peak rates are observed in the middle/end of the active/awake phase (Figure 1 B); similar to glycolysis and glucose oxidation, oscillations in glycogen and triglyceride synthesis persist in ex vivo perfused hearts and are severely attenuated or abolished following genetic ablation of the cardiomyocyte circadian clock.14,15 These findings have led to speculation that the heart increases nutrient storage towards the end of the active period following successful forage for food in anticipation of the upcoming sleep-phase fast. Interestingly, both protein synthesis and degradation processes (ubiquitin-proteasome system and autophagy) are elevated at the beginning of the sleep phase (Figure 1 C), and initial studies suggest that these rhythms are in part dependent on the cardiomyocyte circadian clock.16,20
Protein turnover utilizes large quantities of ATP, leading to the hypothesis that this temporal partitioning is advantageous because it (1) spares ATP for contractile function during the active/awake phase, (2) attenuates protein synthesis during the active period when various prohypertrophic stimuli (e.g., blood pressure, epinephrine, amino acids) are elevated, and (3) allows replacement of myocardial proteins that were damaged during the active/awake period (when oxidative metabolism, respiration rate, and oxidant/toxin consumption from diet is increased) in anticipation of the upcoming active/awake period. Likewise, phospholipid synthesis is increased during the sleep phase, possibly in an attempt to replace recently damaged/oxidized lipids.14 Interestingly, previous studies indicate that the heart is resistant to oxidative stress at the beginning of the sleep phase.21 One possible explanation is that increased antioxidant potential at this time (as observed in extra-cardiac tissues) attenuates misfolding or oxidative damage of proteins and other cellular constituents (Figure 1 C). Thus, temporal control of cardiac metabolism appears to play multiple important roles, ranging from the partitioning of ATP between energetically demanding processes to repair of damaged cellular constituents.
Disrupted Metabolic Rhythms as a Potential Mediator of Cardiac Dysfunction
In some ways, the heart is analogous to a car engine, burning fuel in order to provide the work/force required for other components of the vehicle to function. Indeed, the failing heart has previously been described as an engine out of fuel.22 Timing is critical for any engine, as improper timing of fuel ignition adversely affects performance. In addition, the lifespan of the engine is greatly increased through regular preventive maintenance. Metabolic rhythms in the heart serve both timing and preventive maintenance functions. Increased glucose utilization during the active/awake period likely ensures that sufficient ATP is available to meet energetic demands associated with elevated contractile function, while increased protein turnover during the sleep period prevents accumulation of damaged proteins and organelles (e.g., mitochondria), ensuring maintenance of cardiac function. Such concepts suggest that an impairment in metabolic rhythms, either mistiming (i.e., phase shift) or amplitude attenuation, would hinder cardiac performance and ultimately precipitate cardiac dysfunction. Evidence exists to support this theory, including findings that (1) genetic and/or environmental disruption of circadian clocks leads to cardiac dysfunction, and (2) circadian clocks are altered during various cardiometabolic diseases. Herein follows an overview of this evidence.
Whole-body metabolic rhythms diminish with age and are abolished completely as an animal approaches death (by natural causes).23 Interestingly, germline genetic ablation of circadian clock function through knockout of BMAL1 results in an accelerated aging phenotype associated with loss of biological rhythms at multiple levels, particularly metabolism.24 Serial echocardiography revealed development of dilated cardiomyopathy in BMAL1 knockout mice, which correlates with decreased lifespan.25 Underscoring the importance of the clock in the heart, cardiomyocyte-specific BMAL1 ablation recapitulates both cardiac dysfunction and decreased lifespan observed in germline knockout mice.19 Similarly, genetic disruption of the timing of the circadian clock or circadian clock output (as seen in tau mutant hamsters and DBP/HLF/TEF triple knockout mice, respectively) results in cardiac dysfunction.26,27 Circadian clocks are also disrupted through changes in environmental/behavioral factors such as lighting, food consumption, and physical activity at the wrong time of day. Indeed, shift workers exhibit impaired circadian rhythms at multiple levels and have an approximate 2-fold increased risk of developing cardiometabolic disease.28,29
The circadian clock in the heart is attenuated during pressure overload and hypertension-induced hypertrophy as well as ischemia/reperfusion.3032 In contrast, this molecular mechanism is phase shifted during uncontrolled type 1 diabetes mellitus.33 Despite these observations, to date no studies have directly assessed (through flux measurements) whether cardiac metabolic rhythms are impaired or altered during disease states. However, indirect evidence supports this idea. For example, time-of-day-dependent rhythms in expression of genes encoding for metabolic proteins (e.g., glucose transport 4) were markedly attenuated in the heart following pressure-overload induced hypertrophy.12 Should these perturbations in gene expression oscillations translate to metabolic flux, the concept of metabolic inflexibility as a contributing factor in cardiac dysfunction (which usually considers one or more chronic disease states) could lead to a whole new paradigm: that is, the possibility that disease states abolish time-of-day-dependent oscillations in metabolism.
Temporal partitioning of cardiac metabolism has emerged as a fundamental cornerstone in cardiac biology, governed in large part by the autonomous cardiomyocyte circadian clock. What remains to be fully explored are the molecular links between the circadian clock and metabolic processes as well as the extent to which metabolic rhythms are perturbed during disease states and their precise contribution towards the pathogenesis of cardiac dysfunction. Similarly, the extent to which the circadian clock synergizes with neurohumoral influences (e.g., norepinephrine, insulin) to modulate daily metabolic processes remains to be fully elucidated.
- The heart exhibits profound time-of-day-dependent oscillations in glucose, fatty acid, and protein metabolism.
- The cardiomyocyte circadian clock governs cardiac metabolic rhythms.
- Disruption of circadian clocks leads to impaired metabolic rhythms and cardiac dysfunction.